Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is used to detect and localize antigens (e.g., proteins) in cells or tissue sections by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

ChemPartner has accumulated extensive hands-on working experience in histopathology that could provide strong support to oncology, immunology, inflammation, neuroscience, metabolic & cardiovascular diseases, toxicology and discovery of large/small molecule drugs. ChemPartner is dedicated to providing high-quality and efficient service for IHC assay development, protocol validation, single/double staining, image collection and data analysis/interpretation. For non-commercial antibodies, ChemPartner also could provide full assay development for validation and optimization before formal study.

  • Equipment:  IHC autostainer (Dako AS Link48),  Digital Image Scanner (Aperio ScanScope XT ss001310), Nikon Image Collection Microscope, etc
  • Data analysis software: ImageScope (Aperio), Image Pro Plus, Image J and GraphPad Prism 5, etc.
  • Antibodies with established methods: already established IHC methods for ~110 antibodies, i.e.
    • Oncology: Ki67, EGFR, p-EGFR, HER2, HER3, HER4, MEK1/2, Akt, p-Akt, ERK, p-ERK, PTEN, HGF, IGFR, c-Met, p-c-Met, c-Myc, n-Myc, E-Cadherin, p-Cadherin, ER, CD31, HIF 1 alpha, Cleaved Caspase-3, VEGF, etc.
    • Immunology: CD3, CD4, CD8, Foxp3, PD-1, PD-L1, CTLA-4, Iba-1, MPO, etc.
    • Neuroscience: Tyrosine hydroxylase, MBP, MOG, Doublecortin, NG2, GFAP, etc
  • Tissue types: FFPE and frozen samples of cells and tissue, tissue microarray (TMA)

    Representative Images of IHC staining

    Representative Images of IHC Double staining

  • Deliverable:

    • Raw data together with analyzed data in MS Excel format
    • Ordinary Images in JPEG format or scanned images in SVS format
    • Project update and summary in MS PowerPoint format
    • Final report in MS Word format (including  Purpose, Methods, Results and Data Analysis)

    Sample Submission:

    • Test samples could be submitted
    • FFPE blocks of tissues or cells
    • Oct-embedded samples
    • Tissues and cells fixed in formalin
    • Fresh frozen samples
  • Immmunofluorescence (IF)
    Immunofluorescence (IF) staining is used to detect and localize antigens (e.g., proteins) in cells or tissue sections by using of fluorescent dye-conjugated antibodies or non-antibody methods of fluorescent staining (eg. DAPI). Compared with immunohistochemistry (IHC), IF is more widely used to detect co-localization of multiple targets with different fluorescent dyes.

    ChemPartner offers high-quality and efficient service for IF full assay development, protocol validation, single/double staining, image collection and data analysis/interpretation.

    • Equipment:  IHC autostainer (Dako AS Link48), Fluorescence microscope with 100x oil lens (Zeiss, AXIO Scope A1), etc.
    • Imaging software: ZEN 2011 (Zeiss), Image Pro Plus, Image J, etc.
    • Antibodies with established methods:

    Almost all bright-field immunohistochemistry (IHC) staining protocols can be converted to IF staining with fluorescent antibodies.

    • Tissue types: FFPE and frozen samples of cells and tissue, TMA

      Representative Images of IF staining

      Representative Images of IF Double staining

    Deliverable:

    • Raw data together with analyzed data in MS Excel format
    • Images in JPEG format
    • Project update and summary in MS PowerPoint format
    • Final report in MS Word format (including  Purpose, Methods, Results and Data analysis)

    Sample Submission:

    Test samples could be submitted

    • FFPE blocks of tissues or cells
    • Oct-embedded samples
    • Tissues and cells fixed in formalin
    • Fresh frozen samples
    Fluorescent in situ Hybridization (ISH)
    In situ hybridization (ISH) is a type of hybridization that used a labeled complementary DNA, RNA or modified nucleic acids strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or cell. RNA ISH can help define the spatial-temporal patterns of gene expression within cells and tissues, and FISH could be used to detect chromosomal alterations.

    ChemPartner offers high-quality and efficient service for FISH/RNA ISH assay development, staining, image collection and data analysis/interpretation. So far, ChemPartner has handled hundreds of tumor samples for FISH/RNA ISH study and accumulated extensive hands-on working experience with FISH/RNA ISH troubleshooting.

    • Equipment: Hybridization Oven (Abbot StatSpin Thermobrite/ACD HYBEZ), Fluorescence microscope with 100x oil lens (Zeiss, AXIO Scope A1), Digital image scanner, etc.
    • Imaging software: ZEN 2011 (Zeiss), ImageScope (Aperio), Image Pro Plus and Image J
    • Tissue types: FFPE and frozen samples of cells and tissue, TMA

      Representative Images of FISH/RNA ISH staining

  • Deliverable:

    • Raw data together with analyzed data in MS Excel format
    • Ordinary Images in JPEG format or scanned images in SVS format
    • Project update and summary in MS PowerPoint format
    • Final report in MS Word format (including  Purpose, Methods, Results and Data analysis)

    Sample Submission:

    Test samples could be submitted:

    • FFPE blocks of tissues or cells
    • O.C.T-embedded samples
    • Tissues and cells fixed in 10% NBF or 4% PFA
    • Fresh frozen samples