Target-Specific Assays – Antibody Targets

ID

Target

Receptor Ligand Binding Blocking

Cell-based Functional Readout

1

PD1

PD1/PD-L1

PD1/PD-L2

T cell activation (PBMC + CHOK1/OS8/PD-L1)

Mixed Lymphocyte Reaction

Jurkat NFAT luciferase reporter assay

2

PD-L1

PD-L1/PD1

PD-L1/B7.1

T cell activation (PBMC + CHOK1/OS8/PD-L1)

Mixed Lymphocyte Reaction

Jurkat NFAT luciferase reporter assay

3

CTLA4

CTLA-4/B7.1

CTLA-4/B7.2

T cell activation (PBMC/SEB)

T cell blast / Raji co-culture

4

4-1BB

4-1BB/4-1BBL

T cell activation (CHOK1-OS8)

 NFkB luciferase reporter assay

5

GITR

GITR/GITRL

T cell activation

NFkB luciferase reporter assay

6

LAG3

LAG-3/MHC II

LAG-3/FGL1

LAG-3/LSECtin

NFAT luciferase reporter assay

T cell activation (PBMC/SEB)

Antigen specific T cell activation

7

TIM-3

TIM-3/Phosphatidylserine 

T cell activation (PBMC+CHOK1/OS8 + inducer)

8

OX40

OX40/OX40L

T cell activation 

NFκB reporter assay

9

TIGIT

TIGIT/CD155

TIGIT/CD122

NFAT luciferase reporter assay

10

NKG2A

NKG2A/MHC

NK cell mediated cytotoxicity 

11

CD70

CD70/CD27

Cytotoxicity 

12

CD73

hCD73 ECD-his

CD73 Enzymatic activity

AMP – T cell Suppression 

Internalization 

13

CD47

CD47/SIPRa

Phagocytosis 

Hemagglutination 

14

SEMA4D

SEMA4D/Plexin B1

SEMA4D/Plexin B2

Cell migration 

Cell detachment