Primary Immune Cell Assays
- Whole blood/PBMC stimulation
- Monocyte stimulation DC cell differentiation & stimulation
- T cells (total, CD4 +, CD8+) activation Th subsets differentiation (Th1/2/17/Treg)
- B cell activation
- Antigen recall assay (CMV assay)
- Mixed Lymphocyte Reaction assay
Readouts:
- Cytokine release (ELISA/Luminex )
- FACS
- Proliferation
Detection of Cytokines Release by Luminex
Treg / Th17 / Th1 Cell Polarization
CMV Recall Assay
Mixed Lymphocyte Reaction（MLR）
DC cells are derived from human monocytes by being cultured with GM-CSF and IL-4. DC cells are co-cultured with allogeneic T cells for 5 days.