TIL Subpopulation Screening in Syngeneic Models

TIL Subpopulation Screening in Syngeneic Models

TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca         Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...
Readouts and Methods – Ex Vivo Analysis

Readouts and Methods – Ex Vivo Analysis

Readouts Frequency of immune cell subpopulation Expression of immune checkpoints and markers Soluble factors  Receptor Occupancy     Methods Flow cytometry IHC, IF ELISA, Luminex, CBA ELISpot Western blot, qPCR     Tissues Tumor  Spleen Lymph nodes Peripheral blood...
TIL Subpopulation Screening in Syngeneic Models

Target-Specific Assay Panels – Small Molecule Targets

ID Target Biochemical Assay Cell-based Assays 1 IDO1 IDO1 enzyme activity IFNγ-induced cellular IDO1 in Hela cells;  IDO1 transient/stable transfection in HEK293 cells 2 TDO2 TDO2 enzyme activity TDO2 transient/stable transfection in HEK293 cells 3 ARG1 ARG1 enzyme...
TIL Subpopulation Screening in Syngeneic Models

Target-Specific Assays – Antibody Targets

ID Target Receptor Ligand Binding Blocking Cell-based Functional Readout 1 PD1 PD1/PD-L1 PD1/PD-L2 T cell activation (PBMC + CHOK1/OS8/PD-L1) Mixed Lymphocyte Reaction Jurkat NFAT luciferase reporter assay 2 PD-L1 PD-L1/PD1 PD-L1/B7.1 T cell activation (PBMC +...