siRNA Mediated Target Gene Knockdown

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Knock down target gene by siRNA pool or individual oligos

  • Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively
  • The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay (2D) and/or soft agar colony formation assay (3D)

 

Taqman KD Evaluation

 

Western Blot KD Evaluation (GAPDH as an example)

 

siRNA Anti-Proliferation CTG Assay in Cell Line X

 

siRNA Soft Agar Screen in Cell Line X

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