Functional Assay Panels

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ID

Assay Type

Cell Type

1

Mixed Lymphocyte Reaction (MLR)

Primary human/mouse DC + T cells

2

CMV antigen recall

Primary human T cells

3

T cell activation (anti-CD3/CD28)

Primary human/mouse T cells

4

T cell activation (SEB super-antigen)

Primary human T cells

5

T cell and tumor cell co-culture

Primary human T cells + engineered tumor cells

6

OT-I T cell and OVA tumor cell co-culture

T cells from OT-I mice + OVA-expressing tumor cells 

7

Treg suppressive assay

Primary mouse T cells

8

Treg differentiation

Primary human/mouse T cells

9

Th1 differentiation

Primary human/mouse T cells

10

Th2 differentiation

Primary human/mouse T cells

11

Th17 differentiation

Primary human/mouse T cells

12

M1/M2 Macrophage differentiation

Primary human/mouse macrophages

13

Macrophage phagocytosis

Primary human/mouse macrophages

14

Mouse T cell and M2 Macrophage co-culture 

Mouse T cells + M2 Macrophages

15

NLRP3 activation assay

Mouse BMDM, human THP-1 cells

16

Basophil activation assay

Human whole blood

17

B cell activation and proliferation

Primary human/mouse B cells or whole blood

18

Target specific reporter gene assay (41BB, etc.)

Engineered cell lines

19

Antibody-Dependent Cellular Cytotoxicity (ADCC)

Primary human NK or NK-92-CD16 + target cells

20

NK cell cytotoxicity

Primary human NK or NK-92-CD16 + target cells

21

Complement Dependent Cytotoxicity (CDC)

Complement + target cells

22

ELISpot

Primary human PBMCs or mouse T cells or splenocytes or TILs 

Treated with 

anti-CD3 or peptides or peptide loaded APC or target cells

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