Cell Functional Assays

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Cell Proliferation/Viability

  • CellTiter-Glo cell viability assay
  • CyQuant assay
  • MTT/MTS assays
  • Direct cell counting by HCA
  • Clonogenic assay
  • 14-day long-term proliferation assay

 

3D Growth

  • Soft agar colony formation assay
  • Matrigel 3D assay
  • Ultra-low attachment assay

 

Cytotoxicity

  • LDH release assay

 

Apoptosis

  • FACS (AnnexinV and PI)
  • Caspase 3/7 Glo
  • Cell death detection ELISA

 

Cell Cycle

  • FACS

 

Tumor Cell Migration

  • Transwell Assay

 

Soft Agar Colony Formation Assay


Cell Cycle Analysis

Cell cycle was changed in A549 after cells being treated with Cisplatin for 48h. 

 

Apoptosis Analysis

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