CRISPR/Cas9 Mediated Target Gene Knockout

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Validation of Cas9 Expression by Western Blot

Validation of Cas9 Expression by FACS

Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection)

CRISPR Target Gene Knockout – Phenotypic Study

Knockout of PCNA, an essential gene for cell survival, by Lenti-sgRNA infection, led to scarce colony formation

2D Clonogenic Assay in A549 WT/Cas9 cells following sgCopGFP/sgPCNA virus infection

A549-wt

A549-Cas9

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