Epitope Binning by Octet Red 384

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Classical sandwich assay format was selected for epitope binning using Octet Red 384. About nine bins were generated based on the result.

Classical Sandwich Assay

Sandwich format requirement:

  • Ag reach equilibrium in 5 minutes
  • Disassociation of Ag <50% in 10 minutes (Koff < 10E-03)
  • Ag is a monomer

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