Molecular Pathology: Immunofluorescence (IF)

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Immunofluorescence (IF) staining is used to detect and localize antigens (e.g., proteins) in cells or tissue sections by using of fluorescent dye-conjugated antibodies or non-antibody methods of fluorescent staining (eg. DAPI). Compared with immunohistochemistry (IHC), IF is more widely used to detect co-localization of multiple targets with different fluorescent dyes.

ChemPartner offers high-quality and efficient services for IF full assay development, protocol validation, single/double staining, image collection, and data analysis/interpretation.

  • Equipment: IHC autostainer (Dako AS Link48), fluorescence microscope with 100x oil lens (Zeiss, AXIO Scope A1), etc.
  • Imaging software: ZEN 2011 (Zeiss), Image Pro Plus, Image J, etc.
  • Antibodies with established methods

 

Almost all bright-field IHC staining protocols can be converted to IF staining with fluorescent antibodies.

  • Tissue types: FFPE and frozen samples of cells and tissue, TMA

 

Representative Images of IF Staining

 

Representative Images of IF Double Staining

 

Deliverable:

  • Raw data together with analyzed data in MS Excel format
  • Images in JPEG format
  • Project update and summary in MS PowerPoint format
  • Final report in MS Word format (including purpose, methods, results, and data analysis)

 

Sample Submission:

Test samples could be submitted

  • FFPE blocks of tissues or cells
  • Oct-embedded samples
  • Tissues and cells fixed in formalin
  • Fresh frozen samples

 

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