shRNA Mediated Target Gene Knockdown and Stable Cell Line Generation

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Constitutive and Inducible shRNA Knockdown

  • Constitutive target gene knockdown
  • Tet-inducible target gene knockdown
  • Lentivirus packaging, infection, and stable cell line generation

Verify Inducible Target KD in Stable Cell Lines by RT-Taqman

Verify Target KD in Stable Cell Lines by Western blot

Evaluate Growth Inhibition by Clonogenic Assay

Non-Targeting shRNA

Target A shRNA #1

Target A shRNA #2

Target A shRNA Stable Cell Lines

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