• Concentration determination: spectrophotometry

• Endotoxin level determination: LAL method

• Drug to antibody ratio (DAR) determination: LC-MS, HPLC-HIC, RP-HPLC, etc.

• Purity determination: SEC/ RP/ HIC-HPLC, SDS-PAGE, etc.

• Residues of free drug determination: LC-MS/MS

• Residues of solvent determination: GC

• Site mapping determination: LC-MS

• Sterility test: membrane filtration/direct inoculation

ADC Binding Properties

• Biacore 8K

• Octet Red

• Biotek Plate Reader

• FACS Canto II

Physiochemical Properties

• Biomolecule/Linker-Payload Residue LC-MS

• SEC/HIC/RP/CEX-HPLC

• SDS-PAGE

• CE-SDS/iCIEF

ADC Stability Studies

• Uncle Protein Stability

DAR determination by multiple methodologies

LC-MS

HPLC-HIC

Reverse Phase-HPLC