siRNA Mediated Target Gene Knockdown
Knock down target gene by siRNA pool or individual oligos
Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively
The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay (2D) and/or soft agar colony formation assay (3D)
Taqman KD Evaluation
Western Blot KD Evaluation (GAPDH as an example)
siRNA Anti-Proliferation CTG Assay in Cell Line X
siRNA Soft Agar Screen in Cell Line X
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