Affinity Maturation
Antibody optimization services for leads that bind but not well enough. We combine library-based maturation, CDR engineering and structure-guided design to improve affinity, selectivity and developability together — then confirm every gain in the functional assay that matters to your program.
OVERVIEW
Everything a sponsor evaluating an outsourced biologics CMC partner needs to know — from capacity to compliance.
Apart from antibody humanization and antibody discovery from phage display, ChemPartner’s antibody engineering department also offers sequence optimization and affinity maturation services. We provide customized affinity maturation strategies [random mutagenesis (chain shuffling/error-prone PCR) or site-directed mutagenesis (soft or hard randomization)] based on initial antibody sequences and affinity. Panning and screening strategies are customized and designed for each project to meet the pre-defined criteria/deliverables.
- Kd improvement to sub-nM to pM
- Binding kinetics (off-rate) improvement
- Both scFv and Fab formats may be used to generate CDR mutagenesis libraries (Random or Site-Directed Mutagenesis)
- Libraries are panned against biotinylated Antigen or target cells at decreasing concentrations in solution
- To select for pM binders, off-rate selection method will be employed. Unlabeled antigen is used for competition.
- Enriched clones are produced in 96-well format and final ranking with high throughput Octet RED384
- Best clones identified for full IgG conversion and extensive characterization by SPR assay and cell based functional assays
- Standard timeline with full IgG delivery: 3 months for final KD~nM or 4-5 months for final KD~pM
