Cell Proliferation/Viability

• CellTiter-Glo cell viability assay

• CyQuant assay

• MTT/MTS assays

• Direct cell counting by HCA

• Clonogenic assay

• 14-day long-term proliferation assay

3D Growth

• Soft agar colony formation assay

• Matrigel 3D assay

• Ultra-low attachment assay

Cytotoxicity

• LDH release assay

Apoptosis

• FACS (AnnexinV and PI)

• Caspase 3/7 Glo

• Cell death detection ELISA

Cell Cycle

• FACS

Tumor Cell Migration

• Transwell Assay

Soft Agar Colony Formation Assay

Cell Cycle Analysis

Cell cycle was changed in A549 after cells being treated with Cisplatin for 48h.  

Apoptosis Analysis