• Concentration determination: spectrophotometry
• Endotoxin level determination: LAL method
• Drug to antibody ratio (DAR) determination: LC-MS, HPLC-HIC, RP-HPLC, etc.
• Purity determination: SEC/ RP/ HIC-HPLC, SDS-PAGE, etc.
• Residues of free drug determination: LC-MS/MS
• Residues of solvent determination: GC
• Site mapping determination: LC-MS
• Sterility test: membrane filtration/direct inoculation

ADC Binding Properties

• Biacore 8K
• Octet Red
• Biotek Plate Reader
• FACS Canto II

Physiochemical Properties

• Biomolecule/Linker-Payload Residue LC-MS
• SEC/HIC/RP/CEX-HPLC
• SDS-PAGE
• CE-SDS/iCIEF

ADC Stability Studies

• Uncle Protein Stability

DAR determination by multiple methodologies

LC-MS

HPLC-HIC

Reverse Phase-HPLC