Radiometric
Radiometric assays for targets where a label-free or fluorescent readout will not hold up. Filtration and scintillation proximity formats deliver direct, interference-resistant measurement of binding and enzyme activity — still the reference method for difficult systems.
OVERVIEW
ChemPartner has provided a variety of radiometric assay services to a large number of customers and accumulated a wealth of experience in the last two decades. There are four types of radioisotopes ([3H], [14C], [33P], [35S]) available. The key assay formats include filter plate, SPA beads, Ffashplate, and Cytostar-T. This methodology is mainly used to detect methyl-transferase, transporter metabolite uptake, receptor-ligand binding, and receptor occupancy.
Methyl Transferase Activity Assay
Km Determination of 3H-SAM on HMT Activity Assay
Run-to-Run Correlation on HMT Activity with Flashplate
Ex Vivo Receptor Occupancy
RO/PK Correlation
Equipment
- Microbeta 2
- PlateMate 2×3
