Cell Functional Assays

Phenotypic and functional cell assays that measure biological consequence rather than binding. Proliferation, apoptosis, migration, differentiation and cytokine readouts run in disease-relevant cell systems, configured to the question your program actually needs answered.

Cell Proliferation/Viability

  • CellTiter-Glo cell viability assay
  • CyQuant assay
  • MTT/MTS assays
  • Direct cell counting by HCA
  • Clonogenic assay
  • 14-day long-term proliferation assay

3D Growth

  • Soft agar colony formation assay
  • Matrigel 3D assay
  • Ultra-low attachment assay

Cytotoxicity

  • LDH release assay

Apoptosis

  • FACS (AnnexinV and PI)
  • Caspase 3/7 Glo
  • Cell death detection ELISA

Cell Cycle

  • FACS

Tumor Cell Migration

  • Transwell Assay

Soft Agar Colony Formation Assay

Cell Cycle Analysis

Cell cycle was changed in A549 after cells being treated with Cisplatin for 48h.  

Apoptosis Analysis

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