Cell Functional Assays
Phenotypic and functional cell assays that measure biological consequence rather than binding. Proliferation, apoptosis, migration, differentiation and cytokine readouts run in disease-relevant cell systems, configured to the question your program actually needs answered.
Cell Proliferation/Viability
- CellTiter-Glo cell viability assay
- CyQuant assay
- MTT/MTS assays
- Direct cell counting by HCA
- Clonogenic assay
- 14-day long-term proliferation assay
3D Growth
- Soft agar colony formation assay
- Matrigel 3D assay
- Ultra-low attachment assay
Cytotoxicity
- LDH release assay
Apoptosis
- FACS (AnnexinV and PI)
- Caspase 3/7 Glo
- Cell death detection ELISA
Cell Cycle
- FACS
Tumor Cell Migration
- Transwell Assay
Soft Agar Colony Formation Assay
Cell Cycle Analysis
Cell cycle was changed in A549 after cells being treated with Cisplatin for 48h.
Apoptosis Analysis
