CRISPR-Cas9-Mediated Target Gene Knockout
CRISPR-Cas9 knockout and cell line generation for clean genetic validation. We design and screen guides, isolate and verify clonal lines, and confirm loss of function at protein level — delivering isogenic pairs that make phenotypic comparisons defensible.
Validation of Cas9 Expression by Western Blot
Validation of Cas9 Expression by FACS
Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection)
CRISPR Target Gene Knockout – Phenotypic Study
Knockout of PCNA, an essential gene for cell survival, by Lenti-sgRNA infection, led to scarce colony formation
2D Clonogenic Assay in A549 WT/Cas9 cells following sgCopGFP/sgPCNA virus infection
A549-wt
A549-Cas9
